Application of photodynamic inactivation against pathogens of urinary tract infections

Photodynamic inactivation (PDI) is an alternative to antibiotic therapy method for biocidal action against microorganisms, which can be used lithotripsy and sanitation of the bladder cavities. Objective. Selection parameters application PDI uropathogenic microorganisms. Materials Methods. In this study we bacterial strains isolated from urine samples patients. Differentiation media biochemical plates were identification The sensitivity microorganisms was studied on pure cultures in native urine. photosensitizer “Photoditazine” (50 µg/ml) work, as well Triton X-100 (5 % vol.) applying increase permeability cell wall gram-negative irradiated by a medical laser device “Latus-K” with wavelength 662 nm. To assess effectiveness PDI, values logarithmic decrease colony-forming unit (CFU) calculated. Statistical analysis made Statistica 10.0 Mann-Whitney criterion. Results. 50 uropathogens belonging 18 species 36 Among them, most common S. aureus, E. coli, P. aeruginosa, K. pneumoniae. value CFU gram-positive bacteria ranged 5 6, corresponds 99.999-99.9999% cells sample. For strains, slightly lower 4 5.5, which, nevertheless, 99.99-99.999% bacteria. addition efficiency 46% 99.99% 99% mirabilis, 16% 94% pneumoniae 97% 99.999% aeruginosa. It should noted that affect both Conclusions. may considered